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rat anti canine cd4  (Bio-Rad)


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    Bio-Rad rat anti canine cd4
    Rat Anti Canine Cd4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti canine cd4/product/Bio-Rad
    Average 95 stars, based on 398 article reviews
    rat anti canine cd4 - by Bioz Stars, 2026-03
    95/100 stars

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    Effect of TGF-β1 on Th1 cytokine production and Tregs differentiation in PBMC cultures. (A) IL-2, (B) IFN-γ, and (C) TNF-α concentrations in cell culture supernatant were measured by ELISA. (D) The percentage of CD25 + Foxp3 + cells among CD4 + lymphocytes was measured by flow cytometry. The Wilcoxon signed-rank sum test was used for statistical analysis ( n = 6, * p <0.05).

    Journal: Frontiers in Veterinary Science

    Article Title: Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression

    doi: 10.3389/fvets.2021.656715

    Figure Lengend Snippet: Effect of TGF-β1 on Th1 cytokine production and Tregs differentiation in PBMC cultures. (A) IL-2, (B) IFN-γ, and (C) TNF-α concentrations in cell culture supernatant were measured by ELISA. (D) The percentage of CD25 + Foxp3 + cells among CD4 + lymphocytes was measured by flow cytometry. The Wilcoxon signed-rank sum test was used for statistical analysis ( n = 6, * p <0.05).

    Article Snippet: Subsequently, 1 μg/mL of anti-canine CD4 mouse monoclonal antibody (R&D systems) and 1 μg/mL phycoerythrin (PE)-conjugated anti-canine CD25 mouse monoclonal antibody (Thermo Fisher Scientific) were added and incubated at 25°C for 30 min.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Flow Cytometry

    Effect of TGF-βRII-Ig on Th1 cytokine production and Tregs differentiation of in PBMC cultures in the presence of TGF-β1. (A) IL-2, (B) IFN-γ, and (C) TNF-α in cell culture supernatants were measured by ELISA. (D) The percentage of CD25 + Foxp3 + cells among CD4 + lymphocytes was calculated by flow cytometry. The Wilcoxon signed-rank sum test was used for statistical analysis ( n = 6, * p <0.05).

    Journal: Frontiers in Veterinary Science

    Article Title: Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression

    doi: 10.3389/fvets.2021.656715

    Figure Lengend Snippet: Effect of TGF-βRII-Ig on Th1 cytokine production and Tregs differentiation of in PBMC cultures in the presence of TGF-β1. (A) IL-2, (B) IFN-γ, and (C) TNF-α in cell culture supernatants were measured by ELISA. (D) The percentage of CD25 + Foxp3 + cells among CD4 + lymphocytes was calculated by flow cytometry. The Wilcoxon signed-rank sum test was used for statistical analysis ( n = 6, * p <0.05).

    Article Snippet: Subsequently, 1 μg/mL of anti-canine CD4 mouse monoclonal antibody (R&D systems) and 1 μg/mL phycoerythrin (PE)-conjugated anti-canine CD25 mouse monoclonal antibody (Thermo Fisher Scientific) were added and incubated at 25°C for 30 min.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Flow Cytometry